Both strands utilized the exact same band of inner primers as with respective PCR assays

Both strands utilized the exact same band of inner primers as with respective PCR assays dos.cuatro.step three. Sequencing out-of DNA Having DNA sequencing, this new amplification circumstances extracted from new PCR responses was purified which have the newest Isolate II PCR and you may Gel Kit (Bioline, London, UK) and you will sequenced in a …

Both strands utilized the exact same band of inner primers as with respective PCR assays Read More »